MIA series FCS Molecular Interaction Analyzer for Single-molecule Kinetics
Features
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High-Content Analysis:
Simultaneously quantifies molar concentration, brightness/oligomerization, size, and binding affinity.
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Native-State Characterization:
High background resistance allows direct profiling in physiological samples (cells, plasma, etc.) without purification.
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Ultra-Sensitive Detection:
Requires only tens of microliters at pM–nM concentrations or single-cell levels.
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Automated Data Workflow:
Equipped with Correlation Lite, Acquisition, and Analysis software for automated batch data collection and processing.
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Cost-Effective Operation:
Optimized for use with JLM-Lifetech reagents and consumables to minimize per-test costs.
Specifications
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Concentration Dynamic Range:
10pM-500nM
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Hydrodynamic Radius Range:
<100nm
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Dissociation Constant (KD) Range:
pM-μM
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Single-Molecule Analysis Modalities:
Alternating Laser Excitation Single-Molecule FRET (ALEX-smFRET), Coincidence Analysis, etc.
Application Fields
Pharmaceutical R&D
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Affinity & Kinetics: Detection of binding affinity and chemical kinetic constants for small molecules or biomacromolecules interacting with targets.
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Stability & Metabolic Profiling: In situ stability and metabolic analysis of nanomedicines or biologics in physiological fluids (e.g., plasma, whole blood).
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Biologics Characterization: Analysis of protein denaturation, aggregation, and structural stability for therapeutic biologics.
Cell Biology
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Signal Transduction: Monitoring changes in molecular concentration and biomolecular interactions driven by specific cellular signaling pathways using solution or cell lysate samples.
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Methodological Complement: Serves as a powerful, solution-phase alternative or complement to traditional cell biology techniques such as Co-Immunoprecipitation (Co-IP) and Western Blot.
Chemistry
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Reaction Mechanics: Determination of equilibrium constants and chemical kinetic constants for complex chemical reactions.
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Polymer Physics: Characterization of diffusion coefficients and structural configurations of high polymers.
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Solution Properties: Analysis of buffer characteristics, including ionic strength, concentration, and viscosity.
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Colloid & Hydrogel Dynamics: Assessment of molecular and nanoparticle diffusion behavior within hydrogels and other colloidal matrices.
Biochemistry & Molecular Biology
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Quantification: Determination of the relative and absolute molar concentrations of biomacromolecules like proteins and nucleic acids.
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Intermolecular Affinities: Precise measurement of binding affinity (KD) and kinetic constants for various systems: biomacromolecule-biomacromolecule, small molecule-biomacromolecule, and biomacromolecule-virus/nanoparticle interactions.
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Hydrodynamic Analysis: Measurement of diffusion coefficients and hydrodynamic radii of biomacromolecules.
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Phase & Aggregation Studies: Conformational analysis of protein denaturation/aggregation, biomacromolecular liquid-liquid phase separation (LLPS), and phase transitions in phospholipid bilayers.
Structural Biology
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Dynamic Mechanisms: Utilizing Single-Molecule FRET (smFRET) assays within solutions or cell lysates to resolve the dynamic structure-function mechanisms of biomacromolecules.
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Conformational Kinetics: Detailed analysis of biomacromolecular conformations and the determination of conformational transition kinetic constants.
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Complementary to Structural Tools: The dynamic insights gained from smFRET provide a vital, live-solution complement to the static structure-function data generated by X-ray Crystallography and Cryo-Electron Microscopy (Cryo-EM).
Photophysics
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Probe Characterization: Measurement of photoluminescence quantum yield (PLQY), photobleaching/quenching efficiency, singlet-triplet state transition kinetics, and fluorescence blinking dynamics for novel fluorescent probes or nanoparticles.
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Photoconversion Mechanisms: Investigation of the underlying mechanisms governing molecular photoconversion.
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Nanomaterial Analysis: Size-dependent photophysical property profiling of next-generation nanofluorophores, including quantum dots (QDs), carbon dots, gold nanoclusters, and aggregation-induced emission (AIE) luminogens.
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