MIA series FCS Molecular Interaction Analyzer for Single-molecule Kinetics

An FCS-based ultra-sensitive single-molecule platform for biomolecular interactions and characterization. It quantifies concentration, size, diffusion, and KD within seconds from microliter samples, enabling native-state, homogeneous assay without purification.
Product Description

Features

  • High-Content Analysis:

    Simultaneously quantifies molar concentration, brightness/oligomerization, size, and binding affinity.

  • Native-State Characterization:

    High background resistance allows direct profiling in physiological samples (cells, plasma, etc.) without purification.

  • Ultra-Sensitive Detection:

    Requires only tens of microliters at pM–nM concentrations or single-cell levels.

  • Automated Data Workflow:

    Equipped with Correlation Lite, Acquisition, and Analysis software for automated batch data collection and processing.

  • Cost-Effective Operation:

    Optimized for use with JLM-Lifetech reagents and consumables to minimize per-test costs.

Specifications

  • Concentration Dynamic Range:

    10pM-500nM

  • Hydrodynamic Radius Range:

    <100nm

  • Dissociation Constant (KD) Range:

    pM-μM

  • Single-Molecule Analysis Modalities:

    Alternating Laser Excitation Single-Molecule FRET (ALEX-smFRET), Coincidence Analysis, etc.

Application Fields

Pharmaceutical R&D

  • Affinity & Kinetics: Detection of binding affinity and chemical kinetic constants for small molecules or biomacromolecules interacting with targets.

  • Stability & Metabolic Profiling: In situ stability and metabolic analysis of nanomedicines or biologics in physiological fluids (e.g., plasma, whole blood).

  • Biologics Characterization: Analysis of protein denaturation, aggregation, and structural stability for therapeutic biologics.

Cell Biology

  • Signal Transduction: Monitoring changes in molecular concentration and biomolecular interactions driven by specific cellular signaling pathways using solution or cell lysate samples.

  • Methodological Complement: Serves as a powerful, solution-phase alternative or complement to traditional cell biology techniques such as Co-Immunoprecipitation (Co-IP) and Western Blot.

Chemistry

  • Reaction Mechanics: Determination of equilibrium constants and chemical kinetic constants for complex chemical reactions.

  • Polymer Physics: Characterization of diffusion coefficients and structural configurations of high polymers.

  • Solution Properties: Analysis of buffer characteristics, including ionic strength, concentration, and viscosity.

  • Colloid & Hydrogel Dynamics: Assessment of molecular and nanoparticle diffusion behavior within hydrogels and other colloidal matrices.

Biochemistry & Molecular Biology

  • Quantification: Determination of the relative and absolute molar concentrations of biomacromolecules like proteins and nucleic acids.

  • Intermolecular Affinities: Precise measurement of binding affinity (KD) and kinetic constants for various systems: biomacromolecule-biomacromolecule, small molecule-biomacromolecule, and biomacromolecule-virus/nanoparticle interactions.

  • Hydrodynamic Analysis: Measurement of diffusion coefficients and hydrodynamic radii of biomacromolecules.

  • Phase & Aggregation Studies: Conformational analysis of protein denaturation/aggregation, biomacromolecular liquid-liquid phase separation (LLPS), and phase transitions in phospholipid bilayers.

Structural Biology

  • Dynamic Mechanisms: Utilizing Single-Molecule FRET (smFRET) assays within solutions or cell lysates to resolve the dynamic structure-function mechanisms of biomacromolecules.

  • Conformational Kinetics: Detailed analysis of biomacromolecular conformations and the determination of conformational transition kinetic constants.

  • Complementary to Structural Tools: The dynamic insights gained from smFRET provide a vital, live-solution complement to the static structure-function data generated by X-ray Crystallography and Cryo-Electron Microscopy (Cryo-EM).

Photophysics

  • Probe Characterization: Measurement of photoluminescence quantum yield (PLQY), photobleaching/quenching efficiency, singlet-triplet state transition kinetics, and fluorescence blinking dynamics for novel fluorescent probes or nanoparticles.

  • Photoconversion Mechanisms: Investigation of the underlying mechanisms governing molecular photoconversion.

  • Nanomaterial Analysis: Size-dependent photophysical property profiling of next-generation nanofluorophores, including quantum dots (QDs), carbon dots, gold nanoclusters, and aggregation-induced emission (AIE) luminogens.

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