2×SuperToughProbeqPCR MasterMix(Universal)

The ready-to-use 2×SuperTough Probe qPCR Master Mix features antibody hot-start Taq polymerase and an optimized buffer. It offers high sensitivity, strong signals, robust inhibitor tolerance, fast run compatibility, and single-digit copy detection.
Product Description

Outstanding Efficiency & Sensitivity

Exhibits excellent linearity (R2 = 0.997) across a wide template range (3 X 100-3 X 105), ensuring highly sensitive and stable detection of single-digit copies.

2-Fold Concentration Resolution

Clearly discriminates minimal 2-fold dilution differences with a 100.223% amplification efficiency and excellent linearity (R2 = 0.999).

Exceptional Inhibitor Tolerance

Demonstrates superior tolerance against common PCR inhibitors such as humic acid, SDS, EDTA, and ethanol. It shows no significant amplification inhibition or Ct fluctuations in complex samples, enabling direct crude sample testing to streamline workflows and enhance accuracy.

Broad Sample Compatibility

Delivers lower Ct values compared to the control group across four distinct DNA templates.

Ultra-High Stability

Amplification performance remains fully uncompromised after storage at 37°C for 7 days or following 45 freeze-thaw cycles.

Fast Protocol Compatibility

Compatible with both standard and fast cycling protocols to significantly shorten experimental run times.

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