2×SuperToughSYBRGreen qPCRMasterMix(Universal)

The ready-to-use 2×SuperTough SYBR Green qPCR Master Mix features antibody hot-start Taq polymerase and an optimized buffer. It offers high sensitivity, strong signals, robust inhibitor tolerance, and stable detection down to single-digit copies.

Product Description

Outstanding Amplification Efficiency & Sensitivity

Single-Copy Level Detection

Excellent linearity (R² = 0.999) across a 3 X 10⁰-3 X 10⁶copy range, ensuring stable detection of low-abundance templates down to single-digit coples.

2-Fold Concentration Resolution

Clearly discriminates minimal 2-fold dilution differenceswith an amplification efficiency of 100.716% and excellent linearity (R² = 1).

Exceptional Inhibitor Tolerance & Anti-Interference

Maintains highly stable amplification with no significant inhibition even when common PCRinhibitors (including bilirubin, SDS, EDTA, ethanol, CaCl₂, and urea) are introduced at systemfinal concentrations.

Robust Direct Plant PCR Capability

Successfully amplifies species-specific genes directly from crude leaf tissue samples (processed via simple grinding) of pea, wheat, soybean, rapeseed, lettuce, and maize.

Ideal for convenient, high-throughput screening of non-polysaccharide and non-polyphenolicplant samples without complex pre-treatments.

Broad-Range GC Content Amplification

Demonstrates high amplification efficiency across extreme GC boundaries using humangenomic DNA (gDNA) as a template, performing effectively from high (68.4%) to low (29.4%)GC contents.

Broad Sample Compatibility

Delivers lower C_t values compared to the control group across four distinct types of DNAtemplates, exhibiting exceptional template adaptability.

Ultra-High Stability

Thermal Stability

Amplification performance remains fully uncompromised after storage at 37°C for 7days.

Freeze-Thaw Resistance

Product performance stays perfectly stable even after 45 freeze-thaw cycles, making it highly suitable for repeated laboratory use and long-term storage.

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