2×SuperTough SYBR Green qPCR Master Mix (Universal)

The ready-to-use 2×SuperTough SYBR Green qPCR Master Mix features antibody hot-start Taq polymerase and an optimized buffer. It offers high sensitivity, strong signals, robust inhibitor tolerance, and stable detection down to single-digit copies.
Product Description
Outstanding Amplification Efficiency & Sensitivity
Single-Copy Level Detection

Excellent linearity (R2 = 0.999) across a 3 X 100-3 X 106copy range, ensuring stable detection of low-abundance templates down to single-digit coples.

2-Fold Concentration Resolution

Clearly discriminates minimal 2-fold dilution differenceswith an amplification efficiency of 100.716% and excellent linearity (R2 = 1).

Exceptional Inhibitor Tolerance & Anti-Interference

Maintains highly stable amplification with no significant inhibition even when common PCRinhibitors (including bilirubin, SDS, EDTA, ethanol, CaCl2, and urea) are introduced at systemfinal concentrations.

Robust Direct Plant PCR Capability

Successfully amplifies species-specific genes directly from crude leaf tissue samples (processed via simple grinding) of pea, wheat, soybean, rapeseed, lettuce, and maize.

Ideal for convenient, high-throughput screening of non-polysaccharide and non-polyphenolicplant samples without complex pre-treatments.

Broad-Range GC Content Amplification

Demonstrates high amplification efficiency across extreme GC boundaries using humangenomic DNA (gDNA) as a template, performing effectively from high (68.4%) to low (29.4%)GC contents.

Broad Sample Compatibility

Delivers lower Ct values compared to the control group across four distinct types of DNAtemplates, exhibiting exceptional template adaptability.

Ultra-High Stability
Thermal Stability

Amplification performance remains fully uncompromised after storage at 37°C for 7days.

Freeze-Thaw Resistance

Product performance stays perfectly stable even after 45 freeze-thaw cycles, making it highly suitable for repeated laboratory use and long-term storage.

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